Catalogue
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Sheep anti Mouse Bub1 beta-NT
Catalog number: X1162P$319.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 250 µg |
Host | Sheep |
Species Reactivity |
Mouse |
Application |
Immunohistochemistry Western Blotting |
Background
Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
Synonyms: Mitotic checkpoint kinase MAD3L
Source
Immunogen: Recombinant protein corresponding to amino acids 10-190 of the mouse Bub1b protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis (1-10 µg/ml). Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Mitotic NIH/3T3 cells
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Myrie, K.A., et al. Mutation and expression analysis of human Bub1 and Bub1b in aneuploid breast cancer cell lines. Cancer Lett. 2000, 152, 193-199.
2. Davenport, J.W., et al. The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner. Genomics 1999, 55, 113-117.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q9Z1S0
Species Accession: Mouse
Safety Datasheet(s) for this product:
EA_Sodium Azide |
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" Immunoprecipitation/ Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry." |
!["
Immunoprecipitation/
Western blot analysis
using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow
cytometry."](https://exalpha-7d62.kxcdn.com/ProductImages/X1162P\X1162P-Figure01.jpg)
" Immunoprecipitation/ Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry."