What is BrdU?

BrdU, or 5-bromo-2'-deoxyuridine, is a synthetic nucleoside analog that is commonly used in molecular biology and cell biology research to label newly synthesized DNA. It is incorporated into the DNA of replicating cells during the S phase of the cell cycle. BrdU can be detected using antibodies specific to BrdU, allowing researchers to track cell proliferation or DNA synthesis.

Immunolabeling of mouse small intestine with the X1545K.1 kit.

BrdU labeling is used in a variety of applications, including:

BrdU Assay Kits

To streamline your projects, we have created assay kits for cell proliferation quantification and detection.

BrdU Immunohistochemistry Kit

The BrdU Cell Immunohistochemistry Kit contains all ingredients (antibodies, buffers and detection reagents) needed for the immunohistochemical detection and quantification of the proliferative activity of cells and tissues. The BrdU kit follows an easy-to-use system with reagents titered for success using the provided proven protocol. The kit is best for analysis of cell proliferation and BrdU immunostaining in cells and tissue samples, including formalin-fixed paraffin-embedded tissues.

BrdU Cell Proliferation Assay Kit

The BrdU Cell Proliferation Assay Kit provides the reagents needed for the quantification of cell proliferation using an ELISA set up. This non-radioactive assay is rapid, easy to perform, and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture. For more flexibility, this 2.5-hour protocol includes reagents so that fixed cells can be stored and assayed later. For high-throughput quantification of cell proliferation in a colorimetric format, this validated kit and protocol provides convenience and reliability.

BrdU Chemiluminescent Cell Proliferation Assay Kit

The BrdU Chemiluminescent Cell Proliferation Assay Kit is a non-isotopic immunoassay for quantifying BrdU incorporation in newly synthesized DNA of proliferating cells. Like the Cell Proliferation Assay Kit detailed above, this non-radioactive assay kit provides all necessary reagents, adjusted by our antibody experts for the best signal to noise ration available, to perform chemiluminescent detection and analysis of cell proliferation. This 2-hour assay protocol is high-throughput screening (HTS) compatible, and the chemiluminescent format provides added sensitivity.

Other Cell Proliferation Reagents

As an Absolute Biotech brand, we are proud to combine our cell proliferation research reagents with our sister brands. Other research antibodies, kits, and reagents for cell cycle analysis include:
• FIX&PERM™ Cell Fixation and Permeabilization Buffers from Nordic/Exalpha: With hundreds of cited applications, FIX&PERM is a proven set of flow cytometry reagents that mildly fixes cells and enables simultaneous characterization of intra- and extra-cellular markers, like BrdU.
• Recombinant Anti-BrdU Antibodies from Absolute Antibody: Our sister brand Absolute Antibody specializes in recombinant engineered antibodies, with reduced non-specific staining and 100% biological definition. Absolute Antibody provides a variety of BrdU antibody species and formats available now, and custom engineering options if you can’t find exactly what you want.
• BrdU Antibodies and Kits from LSBio: For further antibody formats kits, LSBio’s BrdU offerings include rabbit polyclonal anti-BrdU antibodies, BrdU Competitive EIA ELISA kits, and more.