Catalogue

Background
Human herpervirus-8 (HHV-8) cyclin is a component of the HHV-8 virus particle. HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

Synonyms: HHV8cyclin

Source

Immunogen: Inclusion Bodies from E. Coli expressing his-tagged full length human cyclin G2 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Applications: Immunoblotting 1-5 ug/ml. Immunoprecipitation 10-20 ug/ml.

Functional Analysis: Immunoprecipitation

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: room temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
Carbone, A., et al. 'Proliferation in HHV-8-positive primary effusion lymphomas is associated with expression of HHV-8 cyclin but independent of p27(kip1).' Am. J. Pathol. 2000, 156, 1209-1215 Product Specific References: 1. Godfrey, A., et al. 'Inhibiting primary effusion lymphoma by leniviral vectors encoding short hairpin RNA.' Blood, 2005, 105, 2510-2518. 2. Sugaya, M., et al. 'Lymphatic dysfunction in transgenic mice expressing KSHV k-cyclin under the control of the VEGFR-3 promoter.' Blood, 2005, 105, 2356-2363. 3. Jarviluoma, A., et al. 'KSHV viral cyclin binds to p27KIP1 in primary effusion lymphomas.' Blood, 2004, 104, 3349-3354.

Protein Reference(s)

Database Name: UniProt

Accession Number: O40946 (KSHV)

Safety Datasheet(s) for this product: