Catalogue

Background
Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.

Synonyms: Ceh-10 homeodomain-containing homolog

Source
Antibody raised in sheep against a recombinant protein corresponding to amino acids 1 to 131 derived from the N-terminal region of the human Chx10 protein conjugated to KLH.

Product
Ammonium sulphate precipitated antibody in PBS with 0.08% sodium azide

Product Form: Unconjugated

Concentration: See vial for concentration

Specificity
Human Chx10 protein

Applications
Detects Chx10 by Western blot at 0.5 to 1 µg/ml. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.

Functional Analysis: Western Blotting

Positive Control: Rat or mouse retinal tissue lysate.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Liu, I.S., et al. Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer. Neuron 1994, 13, 377-393 2. Chen, C.M. & Cepko, C.L. Expression of Chx10 and Chx10-1 in the developing chicken retina. Mech. Dev. 2000, 90, 293-297 3. Ferda Percin, E., et al. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10. Nat. Genet. 2000, 25, 397-401 4. Nittner, D., et al. Synthetic lethality between Rb, p53 and Dicer or miR-17–92 in retinal progenitors suppresses retinoblastoma formation. Nature Cell Biology (2012), 14, 958-965 5. Kay, J.N., et al. Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate. Nature Neuroscience (2011), 14, 965-972 6. Eiraku, M., et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature (2011), 472, 51-56 7. Pierfelice, T.J., et al. Notch3 Activation Promotes Invasive Glioma Formation in a Tissue Site-Specific Manner. Cancer Res., 71, 1115-1125 (2011). 7. Takae Kiyama, Ching-Kang Chen, Steven W Wang, Ping Pan, Zhenlin Ju, Jing Wang, Shinako Takada, William H Klein, Chai-An Mao Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis https://doi.org/10.1186/s13024-018-0287-z 8. Anastasia Marie Litke; Sarah Samuelson; Kerry R. Delaney; Yves Sauvé; Robert L. Chow Investigating the Pathogenicity of VSX1 Missense Mutations and Their Association With Corneal Disease Investigative Ophthalmology & Visual Science December 2018, Vol.59, 5824-5835. doi:10.1167/iovs.18-2549 9. Jin Young Kim, Raehee Park, Jin Hwan J. Lee, Jinyeon Shin, Jenna Nickas, Seonhee Kim, Seo-Hee Cho, Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye, Developmental Biology, Volume 419, Issue 2, 2016, Pages 336-347, https://doi.org/10.1016/j.ydbio.2016.09.001

Protein Reference(s)

Database Name: SwissProt

Accession Number: P58304

Species Accession: Human

Safety Datasheet(s) for this product: