Catalogue

Background
RalB is a member of the Ras-like GTP binding protein family. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene. Ras and Ras-like proteins function as switches, cycling between 2 distinct conformational states, active when GTP is bound and inactive when GDP is bound. Affinity precipitation assays can be used to determine the activation status of Ras and Ras-like family members. This assay is based on the ability of the Ras family effector proteins to interact specifically with the activated GTP-bound form of their target GTPases.

Synonyms: RalB Recombinant G-Protein, G protein

Source

Immunogen: Recombinant GST protein from E. Coli

Product

Product Form: Unconjugated

Formulation: Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol

Concentration: See vial for concentration

Applications
Ral B protein is provided as a functional G-protein and can be used as a Western blot control for antibodies to Ral B protein or in assays to study to protein-protein interactions of Ral B and related proteins with regulatory proteins.

Functional Analysis: Western Blotting

Storage
Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Zhao, Z. & Rivkees, S.A., Tissue-specific expression of GTPase RalA and RalB during embryogenesis and regulation by epithelial-mesenchymal interaction. Mech. Dev. 2000, 97, 201-204 2. Bhullar, R.P. & Yang, S., Immunodetection of ralA and ralB GTP-binding proteins in various rat tissues and platelets. Mol. Cell Biochem. 1998, 179, 49-55 3. Jilkina, O. & Bhullar, R.P., Generation of antibodies specific for the RalA and RalB GTP-binding proteins and determination of their concentration and distribution in human platelets. Biochim. Biophys. Acta 1996, 1314, 157-166

Protein Reference(s)

Database Name: SwissProt

Accession Number: P11234

Species Accession: Human

Safety Datasheet(s) for this product: