Catalogue

Background
The G beta5 protein is a G protein subunit present predominantly in the central nervous system. This protein appears to be expressed in two forms, one found in the brain (39 kDa) and one found in the retina (44 kDa). It has also been found that Gb5 associates tightly with RGS7, a member of the superfamily of negative regulators of G protein signaling. These complexes have been localized to most regions of the brain, whereas G 1-4 exhibit no observable colocalization with RGS7, despite their widespread codistribution throughout similar neuronal networks.

Source
Rabbits were immunized with a unique peptide sequence derived from the D1 blade of the G 5 protein

Product
This antibody is specific for a peptide sequence within the D1 blade which is not found in the G betas 1-4. Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Product Form: Unconjugated

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Plasma membranes from rat cerebrum

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Liang, J.J., et al. 'RGS7 complex formation and colocalization with the Gbeta5 subunit in the adult rat and influence on Gbeta5gamma2-mediated PLCbeta signaling.' J. Neurosci. Res. 2000: 60, 58-64 2. Cabrera, J.L., et al. 'Identification of the Gbeta5-RGS7 protein complex in the retina.' Biochem Biophys Res Commun. 1998: 249, 898-902 3. Watson, A.J., et al. 'A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina.' J. Biol. Chem. 1996: 271, 28154-28160

Protein Reference(s)

Database Name: SwissProt

Accession Number: O14775

Species Accession: Human

Safety Datasheet(s) for this product: