Catalogue

Background
Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1a gives rise to p and/or q-type calcium currents. P/q-type calcium channels belong to the 'high-voltage activated' (hva) group and are blocked by the funnel toxin (ftx) and by the omega-agatoxin-IVA (omega-aga-IVA). They are however insensitive to dihydropyridines (dhp), and omega- conotoxin-GVIA (omega-ctx-GVIA). voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.

Synonyms: CaV2.1; P/Q-type Voltage-Gated Ca2+ Channel; Cacna1

Source

Immunogen: Synthetic peptide derived from the rat 1A calcium channel conjugated to KLH

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat brain lysate, human brain tissue

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Starr,T.V.B et.al 'Primary structure of a calcium channel that is highly expressed in the rat cerebellum' Proc. Natl. Acad. Sci. U.S.A. 88, 5621-5625 (1991) 2. Snutch,T.Pet.al 'Rat brain expresses a heterogeneous family of calcium channels' Proc. Natl. Acad. Sci. U.S.A. 87 (9), 3391-3395 (1990) 3. Yu,A.S et.al. 'Molecular characterization and nephron distribution of a family of transcripts encoding the pore-forming subunit of Ca2+ channels in the kidney' Proc. Natl. Acad. Sci. U.S.A. 89 (21), 10494-10498 (1992) 4. Hansen PB, , et al.'Vascular smooth muscle cells express the alpha(1A) subunit of a P-/Q-type voltage-dependent Ca(2+)Channel, and It is functionally important in renal afferent arterioles.' Circ Res (United States) 87(10) p896-902 (2000) 5. Stephens,G.I. et.al 'The Cav2.1/alpha1A (P/Q-type) voltage-dependent calcium channel mediates inhibitory neurotransmission onto mouse cerebellar Purkinje cells.' Eur J Neurosci 13(10) 1902-12 (2001)

Protein Reference(s)

Database Name: SwissProt

Accession Number: P54282

Species Accession: Rat

Safety Datasheet(s) for this product: