Catalogue

Background
Rabbit anti-human TOB (Transducer Molecule of erbB-2) reacts with a 45 kDa protein originally identified as a molecule that interacts with the ErbB-2 protein, a receptor protein tyrosine kinase (RPTK). TOB does not contain either SH2 or SH3 domains but is homologous with the N terminus of the anti-proliferative BTG-1 protein. Like BTG-1, TOB when exogenously expressed, is able to suppress the growth of NIH3T3 cells. This growth suppression is hindered by the presence of kinase-active p185erbB2.

Source

Immunogen: Synthetic peptide derived from the TOB protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: HeLa cell lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Matsuda, S., et al. 'Tob, a novel protein that interacts with p185erbB2, is associated with anti-proliferative activity.' Oncogene 1996: 12, 705-713

Protein Reference(s)

Database Name: SwissProt

Accession Number: P50616

Species Accession: Human

Safety Datasheet(s) for this product: