Catalogue

Background
GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of -subunits ( i1, i2 and i3)

Source
Rabbits were immunized with a peptide corresponding to amino acids 345-354 of Gi 1 and 346-355 of Gi 2.

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Antigen affinity purified

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: IMR-5 cells or brain

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Li X, et al. Pertussis toxin-sensitive G protein alpha-subunits: production of monoclonal antibodies and detection of differential increases on differentiation of PC12 and LA-N-5 cells. J. Neurochem. 1995, 64, 1107-1117.

Protein Reference(s)

Database Name: SwissProt

Accession Number: P04899

Species Accession: Human

Safety Datasheet(s) for this product: