Catalogue

Background
The product of the DEGS1 gene is a member of the membrane fatty acid (sphingolipid delta 4) desaturase family. Reported to function by inserting double bonds at delta 4 position in fatty acids. DEGS1 is an intracellular enzyme. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. Two splice variants have been identified.

Synonyms: Migration-inducing gene 15 protein, MLD, dihydroceramide desaturase, sphingolipid delta 4 desaturase, DES1, MGC5079, DEGS

Source

Immunogen: Synthetic peptide derived from the human DEGS1 protein

Product

Product Form: Affinity Purified

Formulation: Provided as solution in Tris buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (1:400 starting dilution) and immunohistochemistry (10-15 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: ELISA

Positive Control: Placenta, normal human brain

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Cadena, D.L., et al. 'The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis.; Biochemistry 36:6960-6967(1997). 2. Ternes, P., et al. 'Identification and characterization of a sphingolipid delta 4-desaturase family.; J. Biol. Chem. 277:25512-25518(2002).

Protein Reference(s)

Database Name: UniProt

Accession Number: O15121 (Human)Q5XIF5 (Rat)O09005 (Mouse)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product: