Catalogue

Background
Cyclin-Dependent Kinase 2 (CDK2), also known as p33cdk2 is expressed earlier in the cell cycle than is p34cdc2. Like p34cdc2, p33cdk2 associates with cyclin A in human cells. However, kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

Synonyms: Anti-Cyclin-Dependent Kinase-2, Phosphorylated; p33cdk2

Source
Rabbits were immunized with a synthetic peptide derived from the phosphorylated human cdk2 protein.

Product
This antibody recognizes only phosphorylated cdk2 protein.

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1:2,000 dilution) and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: CDK2 expressed in cells using Baculovirus as vector.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Coates, S., et al. 'A new pathway for mitogen-dependent cdk2 regulation uncovered in p27(Kip1)-deficient cells.' Curr. Biol. 1999, 9, 163-173 2. Lacy, S. & Whyte, P. 'Identification ofa p130 domain mediating interactions with cyclin A/cdk2 and cyclin E/cdk2 complexes.' Oncogene 1997, 14, 2395-2406

Protein Reference(s)

Database Name: SwissProt

Accession Number: P24941

Species Accession: Human

Safety Datasheet(s) for this product: