Catalogue

Background
Sphingolipids and their metabolic products, including ceramide, sphingosine, and sphingosine-1-phosphate (S1P),1 are important signaling molecules in many biological processes. Ceramide has been is one of the key elements regulating apoptotic responses. Ceramide has been reported as a regulator of several protein kinases and phosphatases, including ceramide-activated protein kinase, protein phosphatase, and protein kinase C. Sphingosine, a metabolite of ceramide, might also play a role in mitochondria-mediated apoptosis. Sphingosine inhibits several protein kinases, including protein kinase C and Ca2 -calmodulin-dependent kinase II. S1P regulates many biological processes, including mitogenesis, differentiation, migration, and suppression of apoptosis.

Synonyms: Acylsphingosine kinase, hCERK, Lipid kinase 4, LK4

Source
Rabbits were immunized with recombinant full-length human ceramide kinase protein

Immunogen: Recombinant full-length human ceramide kinase protein

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Antigen affinity purified

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-10µg/ml). Can also be used for ELISA. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: High level expression in heart, brain, skeletal muscle, kidney and liver

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Lamour NF, Chalfant CE. ‘Ceramide-1-phosphate: The missing link in eicosanoid biosynthesis and inflammation.’ Mol Interv. 2005 Dec;5(6):358-67. 2.Kim JW, Inagaki Y, Mitsutake S, Maezawa N, Katsumura S, Ryu YW, Park CS, Taniguchi M, Igarashi Y. ‘Suppression of mast cell degranulation by a novel ceramide kinase inhibitor, the F-12509A olefin isomer K1.’ Biochim Biophys Acta. 2005 Dec 30;1738(1-3):82-90. Epub 2005 Nov 14. 3. Van Overloop H, Gijsbers S, Van Veldhoven PP. ‘Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.’ J Lipid Res. 2006 Feb;47(2):268-83. Epub 2005 Nov 3. 4. Chalfant CE, Spiegel S. ‘Sphingosine 1-phosphate and ceramide 1-phosphate: expanding roles in cell signaling.’ J Cell Sci. 2005 Oct 15;118(Pt 20):4605-12. Review. 5. Mitsutake S, Igarashi Y. ‘Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor.’ J Biol Chem. 2005 Dec 9;280(49):40436-41. Epub 2005 Oct 3. 6. Wijesinghe DS, Massiello A, Subramanian P, Szulc Z, Bielawska A, Chalfant CE. ‘Substrate specificity of human ceramide kinase.’ J Lipid Res. 2005 Dec;46(12):2706-16. Epub 2005 Sep 18. 7.Kim TJ, Mitsutake S, Kato M, Igarashi Y. ‘The leucine 10 residue in the pleckstrin homology domain of ceramide kinase is crucial for its catalytic activity.’ FEBS Lett. 2005 Aug 15;579(20):4383-8.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8TCT0

Species Accession: Human

Safety Datasheet(s) for this product: