Catalogue

Background
Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms.Exalphas rabbit anti-human caspase-7 antibody reacts with the zymogen and with the 20-23 kDa subunit of caspase-7. Cells should be pretreated with 200 ng/ml of deoxyrubicin for one half hour. Exalphas caspase-7 antibody was generated by immunizing rabbits with the aa 186-198 of human caspase-7.

Synonyms: Cysteine-requiring Aspartate Protease-7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3

Source

Immunogen: Rabbits with the aa 186-198 of human caspase-7

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Applications: western blot, use at 2-10 µg/ml

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16 2. Chandler, J.M., et al. Different subcellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver. J. Bio. Chem. 1998, 273, 10815-10818 3. Behrensdorf, H.A., et al. The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7. FEBS Lett. 2000, 466, 143-147

Protein Reference(s)

Database Name: UniProt

Accession Number: P55210

Safety Datasheet(s) for this product: