Catalogue

Background
Yeast Tup 1p-Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP 1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type-specific genes regulated by the alphga2 and a1/alpha2 repressors. Ssn6-Tupl interacts with class I histone deacetylases required for repression, and rfecrfuitment of yeast Tuplp-Ssn6p repressor is associated with localized decreases in histone acetylation.

Synonyms: General transcriptional corepressor TUP1, Glucose repression regulatory protein TUP1, Flocculation suppressor protein, Repressor AER2, TUP1, AAR1, AER2, AMM1, CYC9, FLK1, SFL2, UMR7, YCR084C, YCR84

Source

Immunogen: Recombinant protein derived from the amino acids 1-200 of yeastt TUP1 protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Mav108 cells expressing recombinant TUP1 protein

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Bone, J.R. and Roth, S.Y. 'Recruitment of the yeast Tup1p-Ssn6p repressor is associated with localized decreases in histone acetylation.' J. Biol. Chem 2001, 276, 1808-1813 2. Watson, A.D., et al. 'Ssn6-Tup1 interacts with class I histone deacetylase required for repression.' Genes and Development 2000, 14, 2737-2744 3. Yang, X., et al. 'Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: Coupling protein O-GlcNAcylation to transcriptional repressor.' Cell 2002, 110, 69-80 4. DeSilva, H., et al. 'Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor.' Genetics 1998, 148, 657-667

Protein Reference(s)

Database Name: UniProt

Accession Number: P16649 (Yeast-Saccharomyces cerevisiae)

Species Accession: Yeast

Safety Datasheet(s) for this product: