Catalogue

Background
Specific for 37 kDaTrpE protein. TrpE is a commonly used tag in some protein expression systems. This antibody can be used for analysis of expression of proteins alone or in conjunction with protein specific antibodies. Can also be used as a negative control antibody as it does not react with any known mammalian protein.

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length recombinant TrpE protein and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting of TrpE conjugated proteins (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Not Available

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Chen, P., et al. 'Use of TrpE fusion protein to identify antigenic domains within the BIV envelope protein.' J. Virol. Methods 1994: 47, 331-343 2. Mercy, M.R., et al. 'A new series of trpE vectors that enable high expression of nonfusion proteins in bacteria.' Protein Expr. Purif. 1992: 3, 57-64

Safety Datasheet(s) for this product: