Catalogue

Background
Two types of T cell antigen receptors (TCRs) are now known, the α/β, and the γ/δ heterodimer, which are expressed at the cell surface in association with the CD3 molecular complex. TCR-γ can occur in either disulphide-linked or non-disulphide-linked forms. The γ/δ T-cell receptor is present on a minor subset of T lymphocytes in peripheral blood, thymus, spleen, and lymph node. In man and Mouse, only a small part of the T lymphocytes of the peripheral blood (<5%) is γ/δ. γ/δ T lymphocytes may complement α/β T cells in terms of antigenic specificities, functional capabilities, or sites of action within the body.

Source
11F2 (anti-TCR gamma delta-l) is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Balb/c mice immunized with protein A-Sepharose/anti-CD3/antigen complex.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Clone 11F2 recognizes all molecular forms of the gamma/delta T-cell receptor (TCR) desribed so far

Applications
11F2 is useful for Enzyme Linked Immuno Sorbent Assays, Flow Cytometry, Immunohistochemistry on frozen sections and Immunoprecipitation. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Borst, J., Van Dongen, J., Bolhuis, R., Peters, P., Hafler, D., De Vries, E., Van de Griend, R. (1988). Distinct molecular forms of Human T cell receptor g/d detected on viable T cells by a monoclonal antibody. J. Exp. Med 167 1626-1644 2. Vroom, T., Scholte, G., Ossendorp, F., Borst, J. (1991). Tissue distribution of Human g/d T cells: No evidence for general epithelial tropism. J. Clin. Pathol. 44 1012-1017 3. Bos, J.D., Teunissen, M.B.M., Cairo, I., Krieg, S.R., Kapsenberg, M.L., Das, P.K., and Borst, J. (1990). T-cell receptor gamma delta bearing cells in normal Human skin J. Invest. Dermatol. 94: 37-42. 4. Lanier, L., Ruitenberg, J., Bolhuis, R., Borst, J., Phillips, J., Testi, R. (1988). Structural and serological heterogeneity of g/d T cell antigen receptor expression in thymus and peripheral blood. Eur J Immunol. 18 1985-1992 5. Lanier, L, Serafini, A., Ruitenberg, J., et all. (1987) The gamma T-cell antigen receptor. J Clin Immunol. 7 429 Testi, R., Lanier, L. (1989). Functional expression of CD28 on T cell antigen receptor g/d-bearing T lymphocytes. Eur J Immunol 19 185 6. Lanier, L., Federspiel, N., Ruitenberg, J., Phillips, J., Allison, J., Weiss, A. (1987). The T cell antigen receptor complex expressed on normal peripheral blood CD4­-, CD8- T lymphocytes: A CD3-associated disulfide-linked g chain heterodimer. J Exp Med 165 1076-1094. 7. Voogt, P., Falkenburg, J., Fibbe, W., et al. (1989). Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor-/CD3-, T cell receptor gd+/CD3+ and T cell receptor-a/ß+/CD3+ lymphocytes 142 1774-1780.

Safety Datasheet(s) for this product: