Catalogue

Background
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.

Synonyms: Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate

Source
Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Product
Provided in 60% glycerol, phosphate buffered saline, and 0.1% 2-Chloroacetamide

Product Form: HRP conjugate

Purification Method: Prot A/G

Concentration: See vial for concentration

Applications
Working dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.

Positive Control: A431 cells stimulated with EGF(Exalpha Cat # X1003)

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: N/A

Safety Datasheet(s) for this product: