Catalogue

Background
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Synonyms: Mouse anti phosphotyrosine, PY20, FITC conjugate

Source
Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Immunogen: Phosphotyrosine coupled to carrier protein

Product
Provided as sterile solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3 mM sodium azide

Product Form: FITC

Purification Method: Prot A/G

Concentration: See vial for concentration

Applications
A dilution of 5-10 ug/ml is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Immunofluorescence

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: N/A

Safety Datasheet(s) for this product: