Catalogue

Background
Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin is also present in secondary granules of polymorphonuclear neutrophils (PMN) and is secreted by some acinar cells. The diagnosis and classifiCation of Human breast carcinomas is mainly based on morphology, which has its difficulties and limitations. Specific markers for breast tumors and additional markers, to correlate with the progress of the disease, are needed. Antibodies to Human milk-fat globule (HMFG) membranes are such potential markers for breast carcinomas. HMFG membranes carry differentiation antigens of the apical site of the secretory cell which may be present also on tumor cells.

Source
67D9 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with milkfat globule membranes.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Monoclonal antibodies 67D2, 67D9 and 67F12 bind to the same or overlapping epitopes on an 80 Kd antigen indiCated as MAM-5. On frozen as well as on formalin-fixed lactating mammary gland sections, a very strong reaction in secretion products is observed. Occasionally, preexisting ducts react in some of the mammary tumors, indiCating an enhanced secretory activity in these parts of the mammary gland.

Applications
67D2 is useful for western blot and immunohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. J. Hilkens, F. Buus, J. Hegers, Ph. Hageman2, J. Calafati, A. Sonnenberg and M. Vanderval. Monoclonal Antibodies anti Human Milk-Fat Globule Membranes Detecting Differentiation Antigens Of The Mammary Gland And Its Tumors. Int J Cancer. 1984 34:197-206.

Safety Datasheet(s) for this product: