Catalogue

Background
Tenascin is an extracellular matrix glyoprotein which is found in numerous types of tissues, but most predominately in the nervous system. It has been shown to be upregulated at the edge of healing wounds and in the stroma of many tumors. Three types of tenascin have been identified, tenascin-C, which was the first to be identified, tenascin-R, which is found almost exclusively in the central nervous system, and tenascin-X, a mammalian tenascin found in some connective tissue and around blood vessels.

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with protein preparation from a homogenate of a human mammary tumor and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Detects human tenascin protein by Western blot at 1-5 µg/ml. Can also be used for immunohistochemistry on frozen and paraffin-embedded tissue sections and for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Verstraeten, A.A. et al. Tenascin expression in basal cell carcinoma. Br. J. Dermatol. 1992, 127, 571-574 2. Le Poole, I.C., et al. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br. J. Dermatol. 1997, 137, 171-178 3. Rulo, H.F., et al. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding). J. Dermatol. Sci. 1993, 5, 54-57

Protein Reference(s)

Database Name: SwissProt

Accession Number: P24821

Species Accession: Human

Safety Datasheet(s) for this product: