Catalogue

Background
The human estrogen receptor has a molecular weight of 67 kDa and consists of 6 functional domains (domains A-F). Functional mapping of the estrogen receptor has determined a transcriptional promoting activity in the A/B domain. The hormone-binding domain (E domain) is located towards the carboxy terminal, whereas the DNA-binding domain (C domain) is found in the central portion of the molecule. It has been speculated that the presence in breast cancer cells of truncated forms of the estrogen receptor lacking the hormone-binding domain might promote the uncontrolled growth of the tumor.

Source
Hybridoma produced by the fusion of splenocytes from mice immunized with purified estrogen receptors from calf uterus and mouse myeloma cells.

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Saturated ammonium sulphate precipitation.

Concentration: See vial for concentration

Applications
Antibody can be used for staining of human and mouse frozen and paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: Immunoprecipitation, Western Blotting, ELISA.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Abbondanza, E., et al. 'Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor.' Steroids 1993, 58, 4-12. 2. Santeusanio, G., et al. 'Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule.' Appl. Immunohistochem Mol Morphol. 2000, 8, 275-284. 3. Hori, M., et al. 'Determination of estrogen receptor in primary breast cancer using two different monoclonal antibodies, and correlation with its mRNA expression.' Pathol. Int. 1999, 49, 191-197. 4. Schuler, G., et al. 'Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.' Biol. Reprod. 2002, 66, 976-982.

Protein Reference(s)

Database Name: SwissProt

Accession Number: P03372

Species Accession: Human

Safety Datasheet(s) for this product: