Catalogue

Background
NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.

Synonyms: CD56, NCAM1, Neural Cell Adhesion Molecule

Source
Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.

Immunogen: Extract from KG1a cell line.

Product
Monoclonal antibody clone C5.9 in PBS containing 0.08% azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Immunohistochemistry (2-5 µg/ml, formalin-fixed, paraffin embedded tissues). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Flow Cytometry

Positive Control: Human neuroblastoma

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Muench, Marcus O., et al. Isolation of Definitive Zone and Chromaffin Cells Based upon Expression of CD56 (Neural Cell Adhesion Molecule) in the Human Fetal Adrenal Gland, The Jurnal of ClinicalEndocrinology & Metabolism, 2003, Vol 88(8) Pages 3921-3930,

Protein Reference(s)

Database Name: UniProt

Accession Number: P13591