Catalogue

Background
CD42a or Platelet glycoprotein IX (GP9) is a small, single-chain membrane glycoprotein of 22 kDa found on the surface of human platelets. It forms a noncovalent complex with CD42b, CD42c and CD42d (GPIbα, GPIbβ, and GPV). The GPIb-IX-V complex is the platelet receptor for von Willebrand factor and is known as von Willebrand's factor-dependent adhesion receptor. The CD42a expression is restricted to platelets and megakaryocytes and as a result the Mouse anti Human CD42a antibody reacts with resting and activated platelets, and weakly on monocytes and megakaryocytes.

Synonyms: CD42a

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Flow Cytometry Consult the appropriate Exalpha Negative Control factsheet to determine the amount of antibody to be used as a control for Platelets. Collect blood aseptically by venipuncture into an ACD or EDTA blood collection tube. Important: Within 5 minutes of blood collection, fix blood sample by placing 100 ?l of blood in test tube containing 1 ml of cold (4°C) 1X PBS with 1% paraformaldehyde. Mix by vortexing. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Prior to staining, wash the fixed blood pellet 2X with 1 ml 1X PBS + 0.1% Azide at RT. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Resuspend the pellet in 1 ml of 1X PBS at RT. To a clean labeled test tube add 10 ?l of Exalpha monoclonal antibody. Carefully add 50 _l of the fixed blood suspension to the bottom of the test tube. Vortex and incubate at room temperature for at least 15 minutes and analyzed within 3 hours. Please note: Using this procedure will yield twenty samples for platelet analysis. Select logarithmic (Log) amplification for both Forward (FSC) and Side (SSC) scatters, while collecting data for platelets. See instrument manufacturer’s instructions for Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Detection of platelet activation with monoclonal antibodies and flow cytometry. Fijnheer R., Modderman P.W., Veldman H., et al. Transfusion 1990:30:20-25 2. Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane Du X, Beutler L., Ruan C., Castaldi P.A., and Berndt M.C., Blood 1987:69:1524-1527 3. Structure of the glycoprotein Ib-IX complex from platelet membranes Fox JEB, Aggerbeck L.P., Berndt M.C., J. Biol. Chem. 1988:263:4882-4890 4. Leucocyte Typing IV: White Cell Differentiation Antigens Nieuwenhuis H.K. Report on functional studies. In: Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford: Oxford University Press: 1989:1002-1003.

Protein Reference(s)

Database Name: UniProt

Accession Number: P14770

Safety Datasheet(s) for this product: