Catalogue

Background
Identification of human NK cell antigen expressing the 50 -70 kDa M.W. surface antigen associated with the IgG Fc receptor III on NK cells and Neutrophils. CD16 is expressed on approximately 15% of peripheral blood lymphocytes and is present on all resting NK cells. CD16 may be expressed on CD3 T cells from certain individuals.

Synonyms: CD16

Source

Immunogen: CD16=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with NK lymphocytes

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Prot A/G chromatography

Concentration: See vial for concentration

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacture’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. CD16 on human gamma delta T lymphocytes: expression, function, and specificity for mouse IgG isotypes. Braakman E; van de Winkel JG; van Krimpen BA; Jansze M; Bolhuis RL Cell Immunol 1992 Aug;143(1):97-107 2. Fc gamma RIII activation is different in CD16+ cytotoxic T lymphocytes and natural killer cells. Uciechowski P; Gessner JE; Schindler R; Schmidt RE Eur J Immunol 1992 Jun;22(6):1635-8 3. Involvement of a metalloprotease in spontaneous and phorbol ester-induced release of natural killer cell-associated Fc gamma RIII (CD16-II). Harrison D; Phillips JH; Lanier LL J Immunol 1991 Nov 15;147(10):3459-65 4. CD16- CD56+ natural killer cells after bone marrow transplantation. Jacobs R; Stoll M; Stratmann G; Leo R; Link H; Schmidt RE Blood 1992 Jun 15;79(12):3239-44 5. Natural killer function in flow cytometry: identification of human lymphoid subsets able to bind to the NK sensitive target K562. Vitale M; Zamai L; Neri LM; Manzoli L; Facchini A; Papa S Cytometry 1991;12(8):717-22 6. In vitro responsiveness to interleukins and theophylline of CD16+, CD56- natural killer cells in a patient with chronic granular lymphocyte disorder. Bayle C; Vitte-Mony I; Lang P; Pico J; Hercend T; Bertoglio J Leukemia 1992 May;6(5):470-6 7. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain. Wirthmueller U; Kurosaki T; Murakami MS; Ravetch JV J Exp Med 1992 May 1;175(5):1381-90

Protein Reference(s)

Database Name: UniProt

Accession Number: Q9ULV2

Safety Datasheet(s) for this product: