Catalogue

Background
Catenins are proteins that connect cadherin/β-Catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-Catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed Human αT-Catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-Catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-Catenins. This indiCates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-Catenins to which it shows an overall amino acid identity of 57%. In cells transfected with αT-Catenin cDNA, interaction with β-Catenin was demonstRated by co-immunoprecipitation. The 892_24D2S antibody anti αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis.

Source
892_24D2S is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a C57Bl/6 Mouse immunized with a synthetic peptide corresponding to amino acids 164-177 of the Human αT-Catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.

Product
The vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: The vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
892_24D2S reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.

Applications
892_24D2S is suitable for immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen and paraffin embedded tissue sections, immunoprecipitation and immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Janssens, B., Goossens, S., Staes, K., Gilbert, B., van Hengel, J., Colpaert, C., Bruyneel, E., Mareel, M., and van Roy, F. (2001). alphaT-Catenin: a novel tissue-specific beta-Catenin-binding protein mediating strong cell-cell adhesion, J. Cell. Sci. 114, 3177-88.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q9UI47, Q9UHD2, Q9UI47

Safety Datasheet(s) for this product: