Catalogue

Background
Disruption of the disabled-1 gene (Dab1) results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. Disabled Ð1 acts downstream of reelin in a signalling pathway.

Synonyms: Disabled homolog 1; DAB1

Source
Goats were immunized with a recombinant protein corresponding to amino acids 1-179 of mouse disabled coupled to a GST fusion protein.

Product

Product Form: Unconjugated

Formulation: Provided as 0.2ml sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Immunoblotting at 1-5 µg/ml, Immunoprecipitation at 5-10µg/ml. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: 293 cells transfected with Dab-1 HA tag

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Howell B.W. et.al (1997). EMBO Journal 1,121-132 2. Homayouni,R. et.al (1999) J. Neuroscience 19(17), 7505-7515

Protein Reference(s)

Database Name: UniProt

Accession Number: P97318 (Mouse)

Species Accession: Mouse

Safety Datasheet(s) for this product: