Catalogue

Background
Tested in immunoelectrophoresis, using different antigen/antibody concentration ratio’s the antiserum gives one characteristic precipitin line. In enzyme-immunocytochemical and immunohistochemical staining of concanavalin A in appropriately prepared substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. The entire test system must be free of sodium azide, since it inhibits the enzyme activity.

Source
The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain alpha-D-mannopyranosyl, alpha-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Product Form: HRP

Formulation: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Specificity
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to concanavalin A from jack bean

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRation of the reactants and the sensitivity of the assay arrangement. Cross-reactivity of this antiSerum has not been tested in detail.

Applications
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.