Catalogue

Background
This product is part of the SCICONS™ product line which offers the gold standard in anti-dsRNA antibodies.
The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements. Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies. Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs either produced synthetically or obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450, in the 4-parameter analysis. It cannot be used to determine the concentration of a different type of dsRNA. The only proper standard for each specific application is the purified version of the dsRNA under investigation. Also, the Poly(I:C) control dsRNA can be used for comparison of the outcomes of incubations with the same antibody lot at different time points or for comparison of the outcomes of incubations with different antibody lots.

Synonyms: Double stranded RNA (dsRNA) control/Poly(I):Poly(C)

Source
In vitro synthesized RNA (heteropolymer consisting of unmodified ribonucleotides)

Product
Each vial contains 50 μl of a 1 mg/ml Poy(I:C) solution in RNAse/DNAse-free STE Buffer (0.1 M NaCl, 1 mM EDTA, 50 mM Tris-HCl, pH 7.0). No preservative is added. Low molecular weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of Poly(I:C) LMW is 0.2 kb to 1 kb.

Purity: Absorbance spectrum

Applications
Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We suggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots. The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration.

Functional Analysis: Direct ELISA and Sandwich ELISA (relative activity to reference antigen)

Storage
Store at -20 °C to -80 °C upon arrival, avoid freeze/thaw cycles, store in aliquots.

Product Stability: Shelf Life: 24 months

Shipping Conditions: Ship on blue ice.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.