Catalogue

Source
A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to horseradish peroxidase.

Product
0.5 mg of donkey anti chicken IgY (H+L), conjugated with HRP in phosphate buffered saline‚ pH 7.2‚ 50% glycerol with 0.1% 2-chloroacetamide.

Product Form: HRP conjugated

Purification Method: Egg-yolk derived IgY Affinity Purified

Concentration: See vial for concentration.

Applications
Optimal conjugate working dilutions should be determined for your particular assay conditions. ELISA: recommended dilution: 1:20,000 Blotting: recommended starting dilution: 1:10,000-1:15,000

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Rintala-Maki, Nina,D., Carolyn A. Goard, Colleen E. Langdon, Vanessa E. Wall, Kathryn E.A. Traulsen, Cory D. Morin, Michel Bonin, Leslie C. Sutherland, 2006, Expression of RBM5-related factors in primary breast tissue. J Cell Biochem. 100: 1440 - 1458. 2. Allen, Robert D. III, Mark N. DeZalia and Samuel H. Speck, 2007, Identification of an Rta responsive promoter involved in driving vHV68 v-cyclin expression during virus replication. Virology 365: 250-259. 3. Upton, Jason W. and Samuel S. Speck. 2006. Evidence for CDK-Dependent and CDK-Independent Functions of the Murine Gammaherpesvirus 68 v-Cyclin. J. Virol. 80(24): 11946-11959. 4. van Ginkel, F.W., D. C. Tang, S. L. Gulley and H. Toro, 2009, Induction of mucosal immunity in the avian Harderian gland with a replication-deficient Ad5 vector expressing avian influenza H5 hemagglutinin. Dev. Comp. Immunol. 33: 28-34. 5. Nguyen, H. 2011, Avian Antibodies Specific to Influenza Virus and Technologically Simple Methods of Their Manufacture and Use. US Patent 20,110,166,328. 6. Mohapatra S, Kawahara M, Khan IS, Yannone SM, Povirk LF. 2011. Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis. Nucleic Acids Res. 39(15):6500-10. 7. Thompson, J. E., Galton, B. C., Taylor, C., Boone, A., Heikkila, E., Cliche, D., Culp-stewart, S., Flanagan, J. G., Senchyna, M. 2011. SiRNA Useful to Suppress expression of eIF-5A1. US Patent Application 20110098460. 8. Hale, C.R., Sonali, M., Elmore, J., Pfister, N., Compton, M., Olson S., Resch, A.M., Glover III, C.V.C., Graveley B.R., Terns, R.M., and M.P. Terns. 2012. Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs. Molecular Cell 45:292-302. 9. Swarts, D.C., Hegge, J.W., Hinojo, I., Shiimori, M., Ellis, M.A., Dumrongkulraksa, J., Terns, R.M., Terns, M.P. and van der Oost, J. 2015. Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA. 10. Cieniewicz B, Dong Q, Li G, Forrest JC, Mounce BC, Tarakanova VL, van der Velden A, Krug LT. 2015. Murine gammaherpesvirus 68 pathogenesis is independent of Caspase-1/11 in mice and impairs IL-1? production upon extrinsic stimulation in culture. J. Virol. 89(13):6562-74. 11. Brandon, D.L. and A. Korn. 2016. Immunosorbent analysis of toxin contamination in milk and ground beef using IgY-based ELISA. Food and Agricultural Immunology 27: 1- 2 12. Comstock, S.S., Maleki, S.J. and S.S. Teuber. 2016. Boiling and frying peanuts decreases soluble peanut (Arachis hypogaea) allergens Ara h 1 and Ara h 2 but does not generate hypoallergenic peanuts. Plos One 11(6):e0157849 13. Gilgunn S, Millán Martín S., Wormald MR,, Zapatero-Rodríguez J, Conroy PJ, O'Kennedy RJ, Rudd PM, Saldova R. 2016. Comprehensive N-Glycan Profiling of Avian Immunoglobulin Y. PLoS One 11(7):e0159859. 14. Punwani D, Kawahara M, Yu J, Sanford U, Roy S, Patel K, Carbonaro DA, Karlen AD, Khan S, Cornetta K, Rothe M, Schambach A, Kohn DB, Malech HL, McIvor RS, Puck JM, Cowan MJ. 2017. Lentivirus Mediated Correction of Artemis-Deficient Severe Combined Immunodeficiency. Hum Gene Ther. 28(1):112-124.

Safety Datasheet(s) for this product: