Catalogue

Background
CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.

Source
BRA4F1 is a mouse IgM monoclonal antibody derived from the fusion of SP2.0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with K562 cells.

Product
Each vial contains 100 µl 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. The product is delivered in lyophilised format.

Formulation: Each vial contains 100 µl 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. The product is delivered in lyophilised format.

Specificity
The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).

Applications
The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Chorváth B , Sedlák J, Suliková M, Plesková I, Augustínová M, Duraj J. Monoclonal antibodies (series Bra-) of broad (non-lineage) or restricted (myelomonocytic) reactivities elicited with granulocytes or K 562 cells. Neoplasma. 1989;36(6):633-42. 2. CD

Protein Reference(s)

Database Name: UniProt

Accession Number: UniProt: P22083 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: