Catalogue

Background
Alkylphenols are used as starting material for the production of surface active substances, detergents, pesticides, paints, textile and paper. It is released during the production and use of these products. Alkylphenols are recognized as endocrine disruptors, in particular estrogenic endocrine disruptors.

Source
Rabbit IgG purified by caprylic acid extraction.

Immunogen: BSA-C8-alkylphenol conjugate

Product
Each vial contains 0.5 ml 0.8 mg/ml of affinity purified polyclonal antibody in phosphate buffered saline (pH 7.2) containing 0.02% sodium azide.

Specificity
Target: Alkylphenols (a family of comparable compounds; as an example nonylphenol is given), CAS no.: 104-40-5 The antibody was tested for specificity using 2 conjugates: BSA-C6-AP and BSA-C8-AP

Species Reactivity: The antibody was tested for specificity using 2 conjugates: BSA-C6-AP and BSA-C8-AP Cross Reactivity BSA-C6-AP BSA-C8-AP 4-Octylfenol 100% 100% 4-tert-Butylphenol 67% 27% 2-sec-Butylphenol 8% 1% 4-pentylphenol 1167% 2000% 4-n-heptylphenol 142% 133% 4-n-propylphenol 500% 250% 2-n-propylfenol 0.20% 0% 4-Isopropylphenol 333% 100% 4-n-hexylphenol 667% 1667% 4-chloro-2-cyclo-hexylphenol 0% 1% nonylphenol(tech) 20% 134% 4-n-nonylphenol 42% 40% bisphenolA 4% 7% 4-cumylphenol n.d. 10% 4,4'-(ethylidene) bisphenol n.d. 10% Bisphenol-A n.d. 0,1% Bisphenol A diglycidyl ether n.d. 1% 4,4'cyclohexylidene bisphenol n.d. 0,1% Bis-(4-hydroxyphenyl)-methane n.d. 1%

Applications
The antibody may be used in ELISA and IAC. For ELISA, the recommended dilution is 1:200, depending on the conjugate used for detection. For positive controls ELISA plates are coated with 400 ng/ml BSA-conjugated C6/C8-alkylphenol.

Storage
This working antibody solution is stable for at least 7 days at 4 °C. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. For long storage purposes to diluted solutions the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.

Shipping Conditions: At ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Meulenberg EP, Koopal K, Rhemrev R. (2005) Immunoassays for alkylphenolic pollutants with endocrine disrupting activity. Intern. J. Environ. Anal. Chem. 85, 871-883.