Catalogue

Background
Human c-myc belongs to a family of 5 myc oncogenes. Amplification of c-myc has been found in several types of human tumors including breast and colon carcinomas. Induction of c-myc in cells grown in the appropriate growth factors or co-expression with survival genes, such as bcl-2 causes cell proliferation. In the abscence of these factors, c-myc expression causes cell death. c-myc gene gives rise to at least two nuclear phosphoproteins of 64kDa and 67kDa, the unphosphorylated form is 49kDa.

Synonyms: cmyc; Myc proto-oncogene protein; transcription factor p64

Source
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human c-Myc protein and mouse myeloma cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Application: Western Blot, Immunoprecipitation (both with and without SDS), Immunofluorescence on frozen sections (c-Myc is a labile protein, therefore preotease inhibitors should be used at all times).

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1) K. Alitala et. al. (1983) Proc. Natl. Acad. Sci., 80: 1707-1711 2)H. Person et.al. (1984) Science 225 687-693

Protein Reference(s)

Database Name: UniProt

Accession Number: P01106 (Human) P01108 (Mouse) P09416 (Rat)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product: