GAL-4, DNA Binding Domain
- Product Code: Z150M
- Size: 100 µg
- Availability:
In Stock - Price (USD): $250
Cat #
Data Sheet
Product Name
GAL-4, DNA Binding Domain
Synonyms
Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast
Host/Source
Mouse
Clone
8C-1
Isotype
IgG1
Product Type
Monoclonal Antibody
Reactivity
Yeast
Applications
Western blot, Immunoprecipitation
Purification
Protein A/G Chromatography
Size
100 µg
Price (USD)
$250
Background
GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Customer Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Database Links:
| SwissProt | P04386 | Yeast |
References
1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. & Sadowski, I. EMBO J. 1993, 12, 1375-1385.2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432.
3. The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.