Acid Ceramidase 1
- Product Code: X2668P
- Size: 10 Miniblots
- Availability:
In Stock - Price (USD): $345
Cat #
Data Sheet
Product Name
Acid Ceramidase 1
Synonyms
N-acylsphingosine amidohydrolase (Acid ceramidase) 1; ASAH1
Host/Source
Rabbit
Isotype
IgG
Product Type
Antigen Immunoaffinity Purified Polyclonal
Reactivity
Human, Rat, Mouse
Applications
Western Blotting, ELISA, Immunohistochemistry
Purification
Antigen Immunoaffiinity Purification
Size
10 Miniblots
Price (USD)
$345
Background
Acid ceramidase is a lipid hydrolyase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. It can also synthesize ceramide from sphingosine and free fatty acids as well. The reverse activity is pH dependent (6.0 vs 4.5, respectively). This suggests that the enzyme may have diverse functions within cells dependent on its subcellular location and the local pH. Recent studies have shown that acid ceramidase activity is aberrantly expressed in several human cancers and that it might be a useful drug target. As an example, inhibitors of enzyme activity have been shown to slow the growth of cancer cells alone or in combination with other established, anti-oncogenic treatments. Aberrant activity has also been shown in Alzheimer?s disease and overexpression may prevent insulin resistant (Type II) diabetes by free fatty acids.
Immunogen
Synthetic peptide derived from the C terminal region of the ASAH1 protein.
Positive Control
Human heart tissue
Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Customer Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target Molecular Weight
44 kDa
Product Image
Image Legend
Immunohistochemical staining of human heart tissue using Acid Ceramidase 1 antibody at 10 µg/ml.
References
1. Park, J.H., et al. 'Acid ceramidase and human disease.' Biochim Biophys Acta 2006; 1758:2133-2138.2. Zeidan, Y.H., et al. 'Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production.' J. Biol. Chem. 2006; 281:24695-24703.
3. Shtraizent, N., et al. 'Autoproteolytic cleavage and activation of human acid ceramidase.' J. Biol. Chem. 2008; 283:11253-11259.