Caspase-12 (NT) (involved in Alzheimers)
- Product Code: X1115P
- Size: 100 µg
- Availability:
In Stock - Price (USD): $245
Cat #
Data Sheet
Product Name
Caspase-12 (NT) (involved in Alzheimers)
Host/Source
Rabbit
Isotype
IgG
Product Type
Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purification
Antigen Immunoaffiinity Purification
Size
100 µg
Price (USD)
$245
Background
Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer?s disease including -secretase presenilin and -amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid- . Caspase-12 is ubiquitously expressed in mouse tissues (1).
Immunogen
Synthetic peptide corresponding to amino acids 2 to 17 of the murine caspase-12
Positive Control
Murine spleen tissue lysate
Formulation
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
Customer Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target Molecular Weight
38.9 kDa
Product Image
Image Legend
Western blot analysis
using anti-Caspase-12
(NT) antibody at 1 µg/ml
on human (1) and mouse
(2) spleen tissue lysate.
Database Links:
| SwissProt | Q6UXS9 | Human |
Product Specific References
This product has been used in:
1. Hetz, C., et al, 'Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein' EMBO Journal 2003, 22, , 5435-542. Hetz, C., et al, 'The Disulfide Isomerase Grp58 Is a Protective Factor against Prion Neurotoxicity' Journal of Neuroscience 2005, 25, , 2793-2802
3. Rao, Rammohan V., et al, 'Coupling Endoplasmic Reticulum Stress to the Cell Death Program' The Journal of Biological Chemistry 2002, 277, 24, 21836-21842
References
1. Nakagawa, T., et al., Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000, 403, 98-103.2. Mehmet, H., Caspases find a new place to hide. Nature 2000, 403, 29-30
3. Van de Craen M, et al., Characterization of seven murine caspase family members. FEBS Lett 1997, 403, 61-9