Exalpha Biologicals, Inc.

	Human TNF (Tumor Necrosis Factor) alpha ELISA Kit
The Exalpha Biologicals, Inc. human Tumor Necrosis Factor alpha (TNFa) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human phospho TNFa in serum, plasma, cell culture supernatants.

Features - Colorimetric assay - HTS compatible format - High sensitivity - 4 deg C storage with long shelf life - Non-radioactive - 5 hour protocol	Performance Summary - Sensitivity: 20 pg/ml - Precision: <10% well-to-well variation - Linearity: Within 5% of expected values
	Ordering Information
	Cat. No. X1851K (pdf)	Size 1 Plate
	For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

Steps	Incubation
1. Equilibrate kit to room temperature	-
2. Add samples and standards to wells	2 hours room temp. (or overnight 4°C)
3. Wash 4x	-
4. Add detector antibody to all wells	2 hours - room temp.
5. Wash 4x	-
6. Add HRP conjugate to all wells	30 minutes - room temp.
7. Wash 4x	-
8. Add substrate to all wells	30 minutes - room temp.
9. Add stop solution to all wells	-
10. Read plate at 450 nm/550 nm	-